Navigate to the Enzymes Dropdown
There are two buttons to toggle the display options for enzymes called “Enzymes”. The first is under the “View” tab of the top banner, and the other can be found under the “Enzymes” tab, both of these function identically.

Select which Enzyme List to Display
Once you have navigated to and selected the “Enzymes” dropdown, you can choose which enzymes to display on your plasmid. By default five options are included “All enzymes”, “Unique cutters”, “Dual cutters” and “Type IIs enzymes”. Alternatively, you can select a custom enzymes list you have created. Once you select which list to display the dropdown will show a checkmark next to the selection and the corresponding list will be shown on the sequence file. Additionally, if you would like a quick overview of the enzymes that do not digest the open sequence, you can select the “Noncutters” button and a pop-up will appear with a list.

Selecting the Display Limit
It is possible to adjust the amount enzymes displayed at one time by selecting the “Display Limit” button at the bottom of the “Enzymes” dropdown. This will give you the option to display 50, 100, 200 or 400 enzymes simultaneously on the file. Note by default this is set to 100.
